The clinical utility of Traditional Chinese Medicine (TCM) herbs/roots extracts in osteoporosis (OP) and osteoarthritis (OA) has been described in multiple reports, but there have been few studies of TCM for preventing bone loss and cartilage degradation simultaneously. Six-month-old female Sprague-...
July 15, 2017
Journal:
American Journal of Translational Research
Author:
Luo Y, Zheng S, Ding Y, Dai Y, Zhou Y, Xiang R, Qvist P, Zheng Q, Bay-Jensen AC, Karsdal MA
Objective: The specific degradation of type II collagen and aggrecan by matrix metalloproteinase (MMP)-9, -13 and ADAMTS-4 and -5 (aggrecanase-1 and -2) in the cartilage matrix is a critical step in pathology of osteoarthritis (OA). The aims of this study were: i) To investigate the relative contrib...
April 24, 2015
Journal:
PLoS One
Author:
He Y, Zheng Q, Jiang M, Christiansen TG, Kassem M, Karsdal MA, Bay-Jensen AC
Purpose: In this study we sought to determine whether a Titin peptide fragment can serve as a clinical biomarker for changes in muscle mass. Methods: Mass spectrometry was used to identify Titin fragment in urine. An antibody against this Titin sequence was raised and used to develop a competitive ...
October 9, 2014
Journal:
Exp Gerontol
Author:
Sun S, Henriksen K, Armbrecht G, Belavý DL, Felsenberg D, Rittweger J, Wang Y, Zheng Q, Nedergaard AF, Karsdal MA
Background: Osteoarthritis (OA) is the most common degenerative joint disease, of which the pathogenesis is inadequately understood. Hypertrophy-like changes have been observed as part of the progression of OA. The aim of the study was to develop and characterize a novel biomarker of chondrocytes hy...
September 22, 2014
Journal:
BMC Musculoskelet Disord
Author:
He Y, Siebuhr AS, Brandt-Hansen NU, Wang J, Su D, Zheng Q, Simonsen O, Petersen KK, Arendt-Nielsen L, Eskehave T, Hoeck HC, Karsdal MA, Bay-Jensen AC
Objectives: Fibrosis is characterized by excessive tissue remodeling resulting from altered expression of various growth factors, cytokines and proteases. We hypothesized that matrix metalloproteinase (MMP) mediated degradation of type IV collagen, a main component of the basement membrane, will rel...
December 13, 2013
Journal:
PLoS One
Author:
Sand JM, Larsen L, Hogaboam CM, Martinez F, Han M, Røssel Larsen M, Nawrocki A, Zheng Q, Karsdal MA, Leeming DJ
Objective: Cathepsin K plays essential roles in bone resorption and is intensely investigated as a therapeutic target for the treatment of osteoporosis. Hence an assessment of the active form of cathepsin K may provide important biological information in metabolic bone diseases, such as osteoporos...
October 6, 2013
Journal:
Clin Biochem
Author:
Sun S, Karsdal MA, Bay-Jensen AC, Sørensen MG, Zheng Q, Dziegiel MH, Maksymowych WP, Henriksen K
Elastin is a signature protein of the arteries and lungs, thus it was hypothesized that elastin is subject to enzymatic degradation during cardiovascular and pulmonary diseases. The aim was to investigate if different fragments of the same protein entail different information associated to two diffe...
June 21, 2013
Journal:
PLoS One
Author:
Skjøt-Arkil H, Clausen RE, Rasmussen LM, Wang W, Wang Y, Zheng Q, Mickley H, Saabye L, Diederichsen ACP, Lambrechtsen J, Martinez FJ, Hogaboam CM, Han M, Larsen MR, Nawrocki A, Vainer B, Krustrup D, Bjørling-Poulsen M, Karsdal MA, Leeming DJ
Extracellular matrix remodelling is a prerequisite for plaque rupture in atherosclerotic lesion. Versican, an extracellular matrix proteoglycan present in normal and atherosclerotic arteries is a substrate for matrix metalloproteinases (MMPs) present in macrophage rich areas. The aim of the current ...
June 3, 2013
Journal:
Int J Clin Exp Med.
Author:
Barascuk N, Genovese F, Larsen L, Byrjalsen I, Zheng Q, Sun S, Hosbond S, Poulsen TS, Diederichsen A, Jensen JM, Mickley H, Register TC, Rasmussen LM, Leeming DJ, Christiansen C, Karsdal MA
\r\nAlzheimer\u0027s disease (AD) is a devastating neurological disease characterized by pathological proteolytic cleavage of tau protein, which appears to initiate death of the neurons. The objective of this study was to investigate whether a proteolytic fragment of the tau protein could serve as b...
May 22, 2013
Journal:
PLoS One
Author:
Henriksen K, Wang Y, Sørensen MG, Barascuk N, Duffin KL, Christiansen C, Zheng Q, Karsdal MA
(AD) is a devastating neurological disease characterized by pathological proteolytic cleavage of tau protein, which appears to initiate death of the neurons. The objective of this study was to investigate whether a proteolytic fragment of the tau protein could serve as blood-based biomarker of cogni...
May 22, 2013
Journal:
PLoS One
Author:
Henriksen K, Wang Y, Sørensen MG, Barascuk N, Suhy J, Pedersen JT, Duffin KL, Dean RA, Pajak M, Christiansen C, Zheng Q, Karsdal MA
Background: The proteoglycan biglycan (BGN) is involved in collagen fibril assembly and its fragmentation is likely to be associated with collagen turnover during the pathogenesis of diseases which involve dysregulated extracellular matrix remodeling (ECMR), such as rheumatoid arthritis (RA) and liv...
May 15, 2013
Journal:
Fibrogenesis Tissue Repair
Author:
Genovese F, Barascuk N, Larsen L, Nawrocki A, Li Y, Zheng Q, Wang J, Veidal SS, Leeming DJ, Karsdal MA
The present study describes the assessment of true formation of type III collagen in different pathologies using a neo-epitope specific competitive Enzyme-linked immunosorbent assay (ELISA) towards the N-terminal propeptide of type III collagen (PRO-C3).\r\n\r\nMETHODS:\r\nThe monoclonal antibody wa...
April 19, 2013
Journal:
Am J Transl Res
Author:
Nielsen MF, Nedergaard AF, Sun S, Veidal SS, Larsen L, Zheng Q, Suetta C, Henriksen K, Christiansen C, Karsdal MA, Leeming DJ
Drug development for dementias is significantly hampered by the lack of easily accessible biomarkers. Fluid biomarkers of dementias provide indications of disease stage, but have little prognostic value, cannot detect early pathological changes, and can only be measured in CSF (cerebrospinal fluid) ...
December 12, 2012
Journal:
Int J Alzheimers Dis
Author:
Wang Y, Sørensen MG, Zheng Q, Zhang C, Karsdal MA, Henriksen K
Drug development for dementias is significantly hampered by the lack of easily accessible biomarkers. Fluid biomarkers of dementias provide indications of disease stage, but have little prognostic value, cannot detect early pathological changes, and can only be measured in CSF (cerebrospinal fluid) ...
December 12, 2012
Journal:
Int J Alzheimers Dis
Author:
Wang Y, Sørensen MG, Zheng Q, Zhang C, Karsdal MA, Henriksen K
Background: Titin is a muscle-specific protein found in cardiac and skeletal muscles which is responsible for restoring passive tension. Levels and functioning of titin have been shown to be affected by cardiac damage. Due to the inherent difficulty of measuring titin levels in vivo in a clinical se...
July 6, 2012
Journal:
J Transl Med.
Author:
Vassiliadis E, Rasmussen LM, Byrjalsen I, Larsen DV, Chaturvedi R, Hosbond S, Saabye L, Diederichsen ACP, Genovese F, Duffin KL, Zheng Q, Leeming DJ, Christiansen C, Morten A Karsdal
Objectives: Ankylosing spondylitis (AS) is a chronic inflammation of the spine and the sacroiliac joints. Current markers of inflammation, such as C-reactive protein (CRP), are reflecting the production of an acute phase reactant rather than tissue specific inflammation, but the use of CRP as a diag...
May 30, 2012
Journal:
Clin Exp Rheumatol
Author:
Skjøt-Arkil H, Schett G, Zhang C, Larsen DV, Wang Y, Zheng Q, Larsen MR, Nawrocki A, Bay-Jensen AC, Henriksen K, Christiansen C, Alxandersen P, Leeming DJ, Karsdal MA
Objectives: Type V collagen has been demonstrated to control fibril formation. The aim of this study was to develop an ELISA capable of detecting a fragment of type V collagen generated by MMP-2/9 and to evaluate the assay as biomarker for ankylosing spondylitis (AS). Design and methods: A fragment ...
May 7, 2012
Journal:
Clin Biochem.
Author:
Veidal SS, Larsen DV, Chen X, Sun S, Zheng Q, Bay-Jensen AC, Leeming DJ, Nawrocki A, Larsen MR, Schett G, Karsdal MA
Background: Collagen deposition and an altered matrix metalloproteinase (MMP) expression profile are hallmarks of fibrosis. Type IV collagen is the most abundant structural basement membrane component of tissue, which increases 14-fold during fibrogenesis in the liver. Proteolytic degradation of col...
October 22, 2011
Journal:
Fibrogenesis Tissue Repair
Author:
Veidal SS, Karsdal MA, Nawrocki A, Larsen MR, Dai Y, Zheng Q, Hägglund P, Vainer B, Skjøt-Arkil H, Leeming DJ
Aim: Liver fibrosis involves excessive remodeling and deposition of fibrillar extracellular matrix (ECM) components, which leads to malfunction of the organ, causing significant morbidity and mortality. The aim of this study was to assess whether levels of a type V collagen fragment, the propeptide ...
August 16, 2011
Journal:
Biomarkers
Author:
Vassiliadis E, Veidal SS, Simonsen H, Larsen DV, Vainer B, Chen X, Zheng Q, Karsdal MA, Leeming DJ
Aim: Arterial extracellular matrix (ECM) remodeling by matrix metalloproteinases (MMPs) is one of the major hallmarks of atherosclerosis. Mimecan, also known as osteoglycin has been implicated in the integrity of the ECM. This study assessed the validity of an enzyme-linked immunosorbent assay (EL...
June 11, 2011
Journal:
Biomark Insights
Author:
Barascuk N, Vassiliadis E, Zheng Q, Wang Y, Wang W, Larsen L, Rasmussen LM, Karsdal MA
Background and aims: During fibrogenesis, in which excessive remodeling of the extracellular matrix occurs, both the quantity of type VI collagen and levels of matrix metalloproteinases, including MMP-2 and MMP-9, increase significantly. Proteolytic degradation of type VI collagen into small fragmen...
June 9, 2011
Journal:
PLoS One
Author:
Veidal SS, Karsdal MA, Vassiliadis E, Nawrocki A, Larsen MR, Nguyen QH, Hägglund P, Luo Y, Zheng Q, Vainer B, Leeming DJ
Objective: Degradation of collagen in the arterial wall by matrix metalloproteinases is the hallmark of atherosclerosis. We have developed an ELISA for the quantification of type III collagen degradation mediated by MMP-9 in urine. Design and methods: A monoclonal antibody targeting a specific MMP-9...
April 30, 2011
Journal:
Clin Biochem
Author:
Barascuk N, Vassiliadis E, Larsen L, Wang J, Zheng Q, Xing R, Cao Y, Crespo C, Lapret I, Sabatini M, Cilleneuve N, Vilaine JP, Rasmussen LM, Register TC, Karsdal MA
OBJECTIVES: Accumulation of extracellular matrix (ECM) components and increased matrix-metalloprotease (MMPs) activity are hallmarks of fibrosis. We developed an ELISA for quantification of MMP-9 derived collagen type III (CO3) degradation. DESIGN AND METHODS: A monoclonal antibody targeting a spec...
July 10, 2010
Journal:
Clin Biochem
Author:
Barascuk N, Veidal SS, Larsen L, Larsen DV, Larsen MR, Wang J, Zheng Q, Xing R, Cao Y, Rasmussen LM, Karsdal MA
OBJECTIVES: Accumulation of extracellular matrix (ECM) components and increased matrix-metalloprotease (MMPs) activity are hallmarks of fibrosis. We developed an ELISA for quantification of MMP-9 derived collagen type III (CO3) degradation. DESIGN AND METHODS: A monoclonal antibody targeting a spec...
July 10, 2010
Journal:
Clin Biochem
Author:
Barascuk N, Veidal SS, Larsen L, Larsen DV, Larsen MR, Wang J, Zheng Q, Xing R, Cao Y, Rasmussen LM, Karsdal MA
Progressive loss of articular cartilage is a central hallmark in many joint disease, however, the relative importance of individual proteolytic pathways leading to cartilage erosion is at present unknown. We therefore investigated the time-dependant release ex vivo of MMP- and aggrecanase-derived fr...
December 18, 2009
Journal:
BMC Res Notes
Author:
Wang B, Chen P, Jensen AC, Karsdal MA, Madsen SH, Sondergaard BC, Zheng Q, Qvist P
Progressive loss of articular cartilage is a central hallmark in many joint disease, however, the relative importance of individual proteolytic pathways leading to cartilage erosion is at present unknown. We therefore investigated the time-dependant release ex vivo of MMP- and aggrecanase-derived fr...
December 18, 2009
Journal:
BMC Res Notes
Author:
Wang B, Chen P, Jensen AC, Karsdal MA, Madsen SH, Sondergaard BC, Zheng Q, Qvist P
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