Objectives: The aim of this study was to investigate the time-dependent effect of insulin-like growth factor-I (IGF)-I on cartilage, evaluated by a novel procollagen type II N-terminal propeptide (PIINP) formation assay. This was performed in a cartilage model.

Methods: Bovine articular cartilage explants were cultured in Dulbecco's modified Eagle's medium (DMEM):F12 in the presence of 0, 0.01, 0.1, 1, 10, or 100 ng/mL of IGF-I. The viability of the chondrocytes was measured by the colorimetric Alamar blue assay. Collagen formation was assessed from the conditioned medium by the PIINP assay. Proteoglycan levels retained in the explants after 22 days of culture were extracted and measured by the sulfated glycosaminoglycan (sGAG) assay.

Results: In the absence of stimulation, PIINP markedly decreased as a function of time (99.4%, p < 0.001). IGF-I dose-dependently stimulated collagen formation and more than 3000% (p < 0.0005) at 100 ng/mL IGF-I at day 20 compared to vehicle control (W/O). IGF-I maintained PIINP at levels comparable to that of day 1. IGF-I dose-dependently protected against proteoglycan loss.

Conclusion: IGF-I dose-dependently maintained cartilage formation. The current developed techniques aid the model to represent a more physiologically relevant model to test novel anabolic drugs for osteoarthritis (OA).

Go to full publication


Please don't hesitate to contact us if you have any questions or other inquiries.

Are you interested in learning more about Nordic Bioscience?
Enter your information in the form and a representative will contact you shortly.

By submitting this form you agree to our terms and conditions.